FungiRESIST™ : An Advanced Platform for Studying Fungal Resistance
Fungicide resistance in plant pathogens is a major challenge for sustainable agriculture. To address this issue, CONIDIA CONIPHY introduces FungiRESIST™, an innovative web solution that combines long-read sequencing technology (ONT) with a bioinformatics pipeline specifically designed for fungicide target genes.
Scientific Objectives
FungiRESIST™ enables:
- Structural characterization of pathogen populations from a single analysis.
- Quantification of alleles and haplotypes associated with resistance mutations.
- Detection of novel target gene modifications, paving the way for evolutionary monitoring.
- Interactive visualization of data for real-time spatial interpretation.
Technology and Methodology
- Long-read sequencing (Oxford Nanopore Technologies): ideal for reconstructing complete haplotypes and analyzing complex genetic regions.
- Optimized pipeline for fungicide target genes: CYP51 (Zymoseptoria tritici), CYTB (Plasmopara viticola), and others.
- Quantitative analysis of known mutations (e.g., S524T, Y461H, G143A) and haplotype combinations.
- Fast turnaround: actionable results in just a few days, compared to several weeks with conventional approaches.
Applications
- Epidemiological monitoring of resistance in fungal populations.
- Decision support for integrated fungicide management.
- Fundamental research on the evolutionary dynamics of resistance mutations.
Example: Zymoseptoria tritici
Complex haplotypes such as D134G-V136A-I381V-Y461H-S524T are tracked to assess the frequency of multi-mutation combinations within populations. This approach provides insights into the correlation between genotype and resistance phenotype.
Why Choose FungiRESIST™?
- Proven expertise: over 15 years of experience in detecting resistance alleles.
- User-friendly interface: analysis, visualization, and interactive mapping.
- Flexibility: compatible with your samples or existing ONT data.
Want to learn more or try the platform?
Our solutions associated with FungiRESIST™
NGS technology
Phytosanitary product testing
Let's work together !
If you have any needs or questions about environmental microbiology, contact us and we'll provide you with the answers you need.