FungiRESIST™ : An Advanced Platform for Studying Fungal Resistance

Scientific Objectives

FungiRESIST™ enables:

• Structural characterization of pathogen populations from a single analysis.
• Quantification of alleles and haplotypes associated with resistance mutations.
• Detection of novel target gene modifications, paving the way for evolutionary monitoring.
• Interactive visualization of data for real-time spatial interpretation.

Technology and Methodology

• Long-read sequencing (Oxford Nanopore Technologies): ideal for reconstructing complete haplotypes and analyzing complex genetic regions.
• Optimized pipeline for fungicide target genes: CYP51 (Zymoseptoria tritici), CYTB (Plasmopara viticola), and others.
• Quantitative analysis of known mutations (e.g., S524T, Y461H, G143A) and haplotype combinations.
• Fast turnaround: actionable results in just a few days, compared to several weeks with conventional approaches.

Applications

• Epidemiological monitoring of resistance in fungal populations.
• Decision support for integrated fungicide management.
• Fundamental research on the evolutionary dynamics of resistance mutations.

Example: Zymoseptoria tritici

Complex haplotypes such as D134G-V136A-I381V-Y461H-S524T are tracked to assess the frequency of multi-mutation combinations within populations. This approach provides insights into the correlation between genotype and resistance phenotype.

Why Choose FungiRESIST™?

• Proven expertise: over 15 years of experience in detecting resistance alleles.
• User-friendly interface: analysis, visualization, and interactive mapping.
• Flexibility: compatible with your samples or existing ONT data.

Want to learn more or try the platform?

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