Our latest scientific publication, “The use of long-read PCR amplicon sequencing to study the evolution of resistance to zoxamide, oxathiapiprolin, and complex III inhibitors in French Plasmopara viticola field populations” (link here) represents a milestone in agricultural research. Published in the Journal of Plant Diseases and Protection, this research highlights the unprecedented effectiveness of the next-generation sequencing (NGS) method based on Oxford Nanopore technology.

The study successfully detected and quantified with remarkable precision the resistance variants to fungicides in P. viticola. By employing this third-generation NGS approach, we observed that nearly all sampled populations in France (23 out of 24) exhibited mutations such as G143A, S34L, and/or the insertion E203-DE-V204 in the cyt b gene, involved in resistance to fungal treatments.

This scientific breakthrough offers considerable benefits for the management of fungicide resistance in vine diseases. The speed and accuracy of long-read PCR amplicon sequencing allow farmers and researchers to respond more effectively to the emergence of resistances, ensuring better crop protection and more sustainable agriculture.

We invite the scientific community and agricultural professionals to consult our full article to  discover how the NGS methodology can transform the fight against plant diseases.

For more information, contact-us.

Conidia-Coniphy: At the Heart of Innovation for Resilient Agriculture.